3D-Fect™ is specifically developed to directly transfect cells cultured in 3D scaffold. 3D-Fect™ is suitable for all kind of scaffolds and cells. 3D matrices not only add a third dimension to cellsí environment, they also allow creating significant differences in cellular characteristics and behavior. Because 3D matrices are routinely used in basic research and therapeutic applications, we have developed 3D-Fect™ transfection reagent specific for scaffolds. In this way, scaffold-based 3D matrices combined with 3D-Fect™/DNA complexes, are colonized by cells to be transfected in a more natural environment. This method allows studying tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth.

• Highly efficient: on cell lines and primary cells
• Specific for 3D scaffolds
• All type of nucleic acids: plasmid DNA, siRNA, shRNA
• Completely biodegradable
• Serum compatible
• Long term protein expression